Evaluation of the Effect of Betulin and Its Alkynyl Derivatives on the Profile of Changes in Gene Expression of the Inflammatory Process of Colorectal Adenocarcinoma Cells (HT-29 Cell Line)

Betulin is a lupane-type pentacyclic triterpene. It characterized by range of biological properties, including anti-cancer and anti-inflammatory activities. also an origin compound for obtaining derivatives with higher activity better bioavailability. Chronic inflammation stimulates the formation pro-cancer microenvironment, promoting tumor growth, cell migration, neoangiogenesis. Many factors, immune system cells, cytokines chemokines released them are involved in this process. Therefore, it has been suggested that optimal target drugs disease could be substances showing activity. The aim study was to indicate direction changes expression genes related inflammatory state colorectal cancer cells promoted betulin its selected alkynyl derivatives. Cytotoxicity assessment carried out using sulforhodamine B (SRB) test, whereas lipophilicity determined reversed-phase thin-layer chromatography (RP-TLC). analysis gene profile colon adenocarcinoma treated performed oligonucleotide microarrays HG-U133A. Based on conducted analysis, can stated 1–3 influence change processes HT-29 lines. highest (FC > 2) were observed HMOX1 (compound 1 vs. control) TMED7 3 mRNAs. An important observation comparison studied compared compounds. Derivative showed greatest similarity control cisplatin. These observations necessity further research impact possible chemical modification